Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni subsp. jejuni |
Crystallization (Comment) | Organism |
---|---|
alpha-subunit the enzyme and its complexes with ATP, and ATP and glycine, sitting drop vapor diffusion method, using 0.1 M Bis-Tris propane:NaOH pH 7.0 and 0.7 M magnesium formate (complex with glycine), or 0.1 M Tris-HCl pH 8.5 and 0.3 M magnesium chloride (complex with ATP), or 0.1 M Na2HPO4-citrate acid pH 4.2, 0.2 M lithium sulfate and 20% (w/v) PEG 1000 (complex with ATP and glycine) | Campylobacter jejuni subsp. jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | Campylobacter jejuni subsp. jejuni | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Campylobacter jejuni subsp. jejuni NCTC 11168 | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni subsp. jejuni | Q9PPK3 | alpha subunit | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | Q9PPK3 | alpha subunit | - |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Campylobacter jejuni subsp. jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | - |
Campylobacter jejuni subsp. jejuni | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Campylobacter jejuni subsp. jejuni |
Synonyms | Comment | Organism |
---|---|---|
Glycyl-tRNA synthetase | - |
Campylobacter jejuni subsp. jejuni |
GlyRS | - |
Campylobacter jejuni subsp. jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Campylobacter jejuni subsp. jejuni |